Have you ever had one of those days where nothing quite works?
Today, Ben, an undergraduate student, started extracting DNA samples and then I finished the protocol. He has successfully extracted DNA samples hundreds of times. So I wasn't worried. I didn't watch what was going on much, just took over when he had to leave at 3:30. It is now 9:30. I have all 115 samples in 95% ethanol.
NO PELLETS = NO DNA . !!!!!!!!!
These samples were irreplaceable. The plants from the field that we phenotyped are gone. Tissue samples are used up and I only have fiber and seed from them buried deep in a pile of other samples to be ginned.
Oh well, I didn't want to do that QTL type study anyway.